Mr Markle publicly took the side of his dad after the 74-year-old claimed he had been told there was “no room” for him to make a speech at Meghan and Harry’s Royal Wedding.
Claiming the Duchess of Sussex, 37, had changed since joining the royals, he said: “Throughout our lives, dad always put Meg first, above me and my sister, but if what he is saying is true it appears she is abusing his love for her.
“It’s selfish, cruel even.
There is no room for a greedy arrogant, deceitful fake humanitarian on this world stage. How dare she? Disgusting
“He gave her everything and now she is giving him nothing but hurt back.
“They say blood is thicker than water, but perhaps the blue blood of the royals now runs through Meg’s veins.
“It’s heartbreaking to see how this has changed her and the devastation it has caused to my family.
“Anyone who saw Meg when she was growing up knew her and my dad were inseparable.
Meghan Markle was branded ‘inhumane’ by her relatives for her alleged treatment of Thomas Markle Snr
“Now it looks as though they may never speak again.”
In his latest chat with the media, Mr Markle Snr complained he had not be given the chance to make a speech as a father of the bride at her wedding on May 19.
He said: “Like any father, I wanted to say a few words.
“I was going to recount the funny story about how she broke the news to me about dating Harry: ‘Daddy, I’ve met a man… Daddy, he’s English… Daddy, he’s a prince.’
“I thought it was a cute story.
“I was going to thank the royal family for welcoming my beautiful daughter into their family.
“But it was a speech that I was never going to be allowed to give. That hurt.”
Mr Markle Snr claimed his relationship with Meghan started changing when she allegedly asked him to stop speaking to another outspoken member of their family, the Duchess’ half sister Samantha Grant.
This demand would have been issued three months ago, after Mrs Grant, 53, publicly criticised the former actress.
Mr Markle Snr said: “Meghan asked me to tell Samantha to stop talking about her and then asked me to stop talking to Samantha.
“I have two daughters. I can’t stop talking to one of them.
“No father should be asked to turn his back on their children.
“I love Meghan but I also love Samantha.”
Mrs Grant didn’t fail to express her opinion hours after the publication of her father’s latest interview.
Thomas Markle Snr’s previous interview allegedly left Meghan and Prince Harry frustrated
Addressing her thoughts directly to Kensington Palace, she wrote on Twitter: “There would be no just reason for her not to want him in her life, as he was good enough to use to make her everything she is.
“She should be grateful. Her diplomacy skills suck and are inhumane.”
She then went as far as issuing a threat to Prince Harry, arguing he will be the “next” person to receive a similar treatment by Meghan.
Mrs Grant wrote: “If you treat your own father like this, Harry is next.
As he breached the royal protocol, Thomas Markle allegedly upset the Queen
“Freezing people out is the best way of hiding the truth.
“There is no room for a greedy arrogant, deceitful fake humanitarian on this world stage.
“How dare she? Disgusting.”
Mr Markle Snr’s previous interviews are said to have stirred relations with the royals, leaving Harry and Meghan allegedly frustrated.
He is said to have upset even the Queen after breaching the royal protocol by revealing to ITV Good Morning Britain’s host Piers Morgan what he claims are Harry’s political view on Brexit and US President Donald Trump.