The Keeping Up With The Kardashians star, 22, instructed for the money to be used to buy more masks and protective gear for medics.
It comes as the make-up mogul has been very vocal about encouraging everyone to stay home as she continues to self-isolate with daughter Stormi.
The doctor, who delivered her daughter Stormi, thanked her: “I am speechless, my eyes are filled with tears of joy and my heart is overwhelmed with gratitude.
“I made a wish to the Universe to gather protective masks for our brave healthcare workers and today my dream came true.”
She continued: “I have never felt more blessed to be a doctor, as helping our brave ER and ICU workers feels just as gratifying as helping my own patients. From the bottom of our hearts, THANK YOU Kylie Jenner. You are my hero.
“This generous donation will help save many precious lives. Our world is a better place with you in it. I love you so much.”
Kylie’s generosity comes as she revealed self-isolation has made Stormi “bored” as they have to find ways to entertain themselves in her $12 million mansion.
“Missing social time … the faster we stay inside the faster we can get back to it.. I hope everyone is taking social distancing serious ??” she wrote.
Kylie was recently urged by the Surgeon General in the United States to reach out to her fans to help stem the coronavirus spread among millennials.
The reality star has an incredible amount of influence with a backing of167 million followers on Instagram.
Jerome Adams said Kylie could become an unlikely coronavirus “hero” by advising her fans to self-isolation.
The reality star duly obliged and took up the coronavirus baton by speaking out on her Instagram story while urging her fans to self-isolate and stay at home until further notice.
Kylie said: “Hey guys. Happy self-quarantine! I know I’ve already been doing my daily reminders about how important it is right now to practice social distancing and self-quarantine.
“I’m going on my ninth day. The coronavirus is a real thing.”
She added: “I listened to the Surgeon General this morning and even though I’ve already been doing my daily reminders, he definitely encouraged me to come on here and talk to you guys so you can see me and hear me.”
Kylie said: “Practice social distancing, self-quarantine.”